Adsorption of Oligonucleotides onto Polyisohexylcyanoacrylate Nanoparticles Protects Them Against Nucleases and Increases Their Cellular Uptake
Identifieur interne : 000362 ( France/Analysis ); précédent : 000361; suivant : 000363Adsorption of Oligonucleotides onto Polyisohexylcyanoacrylate Nanoparticles Protects Them Against Nucleases and Increases Their Cellular Uptake
Auteurs : Christine Chavany [France] ; Tula Saison-Behmoaras [France] ; Trung Le Doan [France] ; Francis Puisieux [France] ; Patrick Couvreur [France] ; Claude Hélène [France]Source :
- Pharmaceutical Research [ 0724-8741 ] ; 1994-09-01.
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Abstract
Abstract: Oligonucleotides can be adsorbed on polyisohexylcyanoacrylate nanoparticles in the presence of hydrophobic quartenary ammonium salts. Oligonucleotides bound to nanoparticles are protected from nuclease attack both in buffer and in cell culture media. Cellular uptake of Oligonucleotides is increased when they are adsorbed onto nanoparticles as a result of the capture of nanoparticles by an endocytic/phagocytic pathway. Intracellular stability towards nucleolytic degradation is increased in the presence of nanoparticles. These results show that nanoparticles can be considered as convenient carriers for the protection and delivery of oligonucleotides to cells.
Url:
DOI: 10.1023/A:1018923301967
Affiliations:
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ISTEX:B9E8556A66469D15B9B939FDD774CDAD9D6497D3Le document en format XML
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Oligonucleotides bound to nanoparticles are protected from nuclease attack both in buffer and in cell culture media. Cellular uptake of Oligonucleotides is increased when they are adsorbed onto nanoparticles as a result of the capture of nanoparticles by an endocytic/phagocytic pathway. Intracellular stability towards nucleolytic degradation is increased in the presence of nanoparticles. 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